The bovine oocyte in vitro maturation was accelerated by adding 10% bovine follicular fluid (bFF) tomaturation medium, but was restrain if adding bFF with concentration of 20% or 30% to maturationmedium.

• 5成熟培养液中添加10%25的牛卵泡液(bFF)对牛卵母细胞的体外成熟有促进作用,但高浓度的bFF(20%25,30%25)则抑制牛卵母细胞的体外成熟。