The binding capacity of Ni2+-IDA Sepharose Fast Flow for His-hepcidin was 30.4mg/ml resin.The disulfide bonds of hepcidin were formed before the fusion protein was cleaved by enterokinase.

• 金属螯合层析中融合蛋白收率不低于90%25，Ni2+-IDA-Sepharose Fast Flow对该融合蛋白的吸附容量为30.；4mg/mL树脂。