The amplified gene was cloned into the pENTR11 vector. With the pENTR11-SLC plasmid and the backbone plasmid pAd/CMV/V5-DEST, the homologous recombination reaction took place in vitro. The reaction mixture was transferred into TOP10 E.

• 方法采用PCR技术从含有SLC基因的质粒上扩增出SLC基因,将PCR产物酶切后连接至pENTR11载体上,再通过pENTR11与腺病毒骨架载体pAd/CMV/V5-DEST之间的同源重组作用将SLC基因片段重组至pAd/CMV/V5-DEST上,最后经293细胞的包装扩增后得到携带SLC基因的重组腺病毒。