The adhesion cells,collected among the MNC ,were divided into seven groups ,add 3 ml RPMI-1640 nutrient medium to every group, then add different combinated cytokines to induce the generation of DC.

• 将获得的单个核细胞(mononuclear cell,MNC)分为7组,每组加入3ml的RPMI-1640培养基,分别添加不同的细胞因子组合诱导DC的生成。