The active anti-AIF scFv was recovered efficiently from inclusion bodies with a refolding yield of approximate 75% confirmed by spectrophotometer.

• AIF4c9scFv表达蛋白在细胞质中主要以不溶性包涵体形式存在,优化后的柱内复性程序可从高浓度的包涵体变性蛋白中高效复性AIF4c9scFv表达蛋白,其回收率可达75%25左右。