The Toxin gene and Bt gene were first transformed into Acer negundo by Agrobacterium-mediated method and needle-sticking method. Transgenic calli and transgenic plants were obtained by PCR and PCR-Southern hybridization analysis.

• 通过农杆菌介导法和针刺法,首次将蜘蛛神经毒蛋白杀虫肽基因Toxin和Bt基因导入复叶槭,经分子生物学检测,获得了复叶槭抗虫转基因愈伤组织和转基因植株。