The SPF chickens were incubated with oil-adjuvanted inactivated AIV vaccine, then the high titer serum was collected and the Immunoglobulin G was extracted with the saturated (NH_4)_2SO_4.And the content of the IgG is 4.090mg/mL.

• 将该病毒抗原制成油佐剂灭活疫苗；免疫SPF鸡；收集高免血清；用(NH_4)_2SO_4法提取血清中的免疫球蛋白G(IgG)；测定蛋白含量为IgG浓度为4.；090mg/mL。