The PCR products were then analyzed by heteroduplex, SSCP and heteroduplex-SSCP analyses separately to testify the effectiveness of each method.

• 方法应用聚合酶链反应(PCR)扩增23个已知的杂合子突变，分别以异源双链、SSCP、异源双链-SSCP法分析突变，对比各方法对突变的检出情况。