The CTAB-guanidinium method,modified CTAB method,and RNAplant Kit method were used to extract the total RNA from the plantlets of apple cultured in vitro,in which contain a lot of carbohydrates and phenolic compound.

• 利用CTAB_异硫氰酸胍法、改良CTAB法和RNAplant试剂盒法,从富含多糖和酚类物质的苹果组培苗继代苗中提取总RNA。