The CK or EMA positive cells could be observed after Serum-free Keratinocyte Medium(K-SFM) consisting of 20ng/ml epidermal growth factor(EGF) treatment. The number of positive cells was correlated to the density of EGF and induced time.

• 含20ng/ml表皮生长因子(Epidermal growth factor,EGF)的无血清角化细胞培养基(Serum-free Keratinocyte Medium,K-SFM)能够在体外诱导犬BMSCs向上皮样细胞分化,分化程度与EGF的浓度及诱导时间有关。