The C-banding and BrdUrd-Hoechst-Giemsa replication pattern in somatic chromosomes in Bufo bufo gargarizans and sister chromatid differential staining technique of the somatic cells in Amphibia are presented in this article.

• 本文报道了中华大蟾蜍(Bufo bufo gargartzans)的C带和BrdUrd-Hoechst-Giemsa复制带,并以中华大蟾蜍为代表确立了两栖类体细胞的姐妹染色单体差别染色(SCD)方法。