The Balb/c mouse was immunized with antigen of purified avian encephalomyelitis virus strain Van Roekel which was adapt for cell culturing. A hybridoma cell line, which can secret monoclonal antibodies against avian encephalomyelitis(AEV), was obtained.

• 利用提纯的禽脑脊髓炎病毒 (AEV)VR株CEF适应毒制备抗原 ,免疫BALB/c小鼠 ,应用杂交瘤技术以间接ELISA筛选获得 1株分泌抗AEV(VR)单克隆抗体的杂交瘤细胞。