The B-D experiment is the main method of examine the pulsation vacuum pressure steam sterilization machine whether safe use,but there are many influence factors in the B-D experiment process.

• 试验是检验脉动真空压力蒸汽灭菌器能否安全使用的主要方法,但B-D试验过程中影响因素多,其阳性检测结果并不都是因灭菌器故障而致。