The AAV vector was cotransfected with two helper plasmids into HEK293T cells by calcium phosphate method,where one helper(pAAV-RC) mediates AAV vector replication,the other(pHelper) has AAV rep and cap genes.

• 方法磷酸钙三质粒共转染方法将pAAV-HRE9-VEGF165、pAAV-Rep/cap、pHelper质粒转染HEK293T细胞,制备2型重组腺病毒相关病毒(rAAV2)。