The 3FLAG-Id3 fusion gene was amplified from p3FLAG-Id3-CMV-10 vector and subcloned into retroviral vector pMX-CITE/IRES-EGFP.

• 以p3FLAG-Id3-CMV-10为模板,用PCR扩增3FLAG-Id3融合基因,将其亚克隆至逆转录病毒载体pMX-C ITE/IRES-EGFP中;