The 1.5kb long anticipated DNA fragment coding for P-450 protein was amplified by PCR, using a pair of low G/C%content primers designed according to CYP6A1 cDNA gene.

• 再以总cDNA为模板，以P-450 CYP6A1 cDNA序列为参考设计一对低G/C%25含量引物，进行PCR扩增，获得1.；5kb左右的预期目的片段。