Tester cDNAs were divided into two groups and ligated to the specific adaptor 1 and adaptor 2R, and then hybridized with normal aloe vera L cDNA twice with two rounds of suppression PCR.

• 将实验方的双链cDNA分成两组，分别与两种不同的接头连接(adaptor 1 and adaptor 2R)。 接着与驱逐方cDNA杂交两次及两轮抑制PCR。