THANK1 34 - 2 85 gene were subcloned into p ET- 11a and m ade a p ET- THANK1 34 - 2 85.BL2 1transformed with p ET- THANK1 34 - 2 85was used to analyze the expression of THANK1 34 - 2 85after induction with1mm ol/L IPTG.

• 重组质粒转化 BL 2 1,以 1m mol/ LIPTG进行诱导表达产物 ,SDS- PAGE分析表达产物 ,对重组蛋白经纯化复性后测定生物学活性。