Subsequently, the explants were placed in turn onto MSBi, MSBz , MSBs containing Kan 50mg/L and Cef 500mg/L to be screened, and the transformants were obtained with transformation frequency amounting to 7.89%.

• 然后依次转入附加Kan 50mg/L+Cef 500mg/L的MSB_1、MSB_2、MSB_3培养基中进行筛选，获得抗性植株。 两个品种的茎尖转化率均为7.；89%25±。