Subsequently, the bitter fractions recognized were separated by reversed phase HPLC on LUNA C18 column and confirmed by sensory testing, three peptides with the most bitter flavor(p2, p4, p8) were obtained.

• 该苦味部分经LUNA C18反相色谱进一步纯化并经感官鉴评，获得三个苦感最强的苦味肽(p2，p4，p8)；