Subsequently, an incomplete dephosphorylation reaction was performed to partially remove the phosphate groups so that the phosphorylation sites of the multi-phosphopeptides might be estimated.

• 随后，不完全的去磷酸化反应使得磷酸基团部分转移，由此，多磷酸化肽的磷酸化位点有可能被检测出来。