Subsequently the isolated proteins were fractionated by polyacrylamide gcl elcctrophoresis and blotted to the filter, and the Fc fragments of IgG on the shects wcre probed with SPA-HRP and identified by enzyme assay.

• 膜上各蛋白质用SPA-HRP探针作酶分析,从而鉴定IgG的Fc片段。