Sperm-mediated DNA transfer by lipofection was performed and the possible affecting factors were researched to optimize the lipofectamine-mediated procedure and conditions.

• 研究了阳离子脂质体介导山羊精子转染外源DNA的方法、效果及其影响因素以确定其最适转染条件,为进一步用此方法生产转基因动物提供参考。