So, we first studied the innate resistant mechanism of HCC Bel7402 cell line, and then we successfully established a 161 fold resistant cell line named Bel5-FU20000 in vitro by continuous stepwise exposure of Bel7402 cells to 5-FU for 6 months.

• 为此，我们首先研究了人肝癌Bel_(7402)细胞株的天然耐药性及其机制，继而又以Bel_(7402)作为亲本细胞，经过体外约6个月的多步骤连续5-FU筛选，得到了对5-FU耐药约161倍的5-FU耐药细胞系Bel_(5-FU20000)，并对其生物学特征及耐药机理进行了研究。