Shoots regeneration frequency increased significantly when cotyledons were preconditioned in MSB5 medium with 1 mg/L 2,4-D and 1 mg/L BA for 3 days and then cultured in MMO medium containing 4.5 mg/L BA.
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释义
将子叶在附加1 mg/L BA和1 mg/L 2,4-D的MSB5培养基中预培养3天,再转入含有4.;5 mg/L BA的MMO培养基中培养,可显著增加芽再生率,并且可将再生周期比对照缩短1/3。
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