Shoot tips of plantlet in vitro were best for callus induction of renewal culture and l/2macro +1.0 mg/L BA+0.1 mg/L 2,4-D was preferable.

• 无菌苗的茎尖最适宜作为‘Jolanba’培养体系更新中愈伤组织诱导的外植体； 诱导愈伤组织的培养基为1/2macro+BA 1.；0mg/L+2，4-D 0