Selective amplification primer combination patterns EcoRI+2/Msel+3 , EcoRI+3/MseI+3 were both suitable to polymorphism analysis of peach genome. The result showed that the AFLP system was can be used for the genome of peach.
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释义
籽预扩增产物稀释20倍用作选择性扩增的模板,选用EcoRI+2/MseI+3、EcoRI+3/MseI+3所组成的128对引物组合进行扩增,扩增条带清晰、多态性高,适合桃基因组分析。
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