Selection was carried out with 1, 3, and 5mg/l ppt (phosphinothricin) and/or 100 and 150mM NaCI in MS based medium solidified with agar followed by its transfer to regeneration medium for shoot induction.
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释义
在转化过程中,在MS固体培养基内分别添加1、3、5 mg/l PPT(phosphinothricin)和/或100、150 mM NaCl作为转化筛选剂。