SPC-A1 cells were inbucated with the pharmaceutical serum containing Hua Tan San Jie Fang and rabbit serum not containing drug separately for 12h,24h,36h,48h,then the expression of TNFR1 mRNA was examined by in situ hybridzation method.

• 化痰散结方含药血清，不含药兔血清分别与人肺癌细胞SPC-A1共同孵育12小时、24小时、36小时、48小时后，运用原位杂交的方法检测人肺癌细胞TNFR1表达的变化。