Results: Cell cultured were typical form of VSMC under light microscope, electron microscope and immunohistochemical test.The cell viability is 95 % in subculture by trypan blue.

• 传至第三代时，用台盼蓝检查细胞传代成活率95%25，光镜、电镜和免疫组化鉴定培养细胞，培养细胞纯度96%25。