Results 103 cases were successfully cultured,among which 98 cases were successful at first time,and 5 cases were later cultured for more cells due to the less number of colone.

• 结果103例一次培养成功,1例培养失败后复穿培养成功。103例中98例原瓶培养即获得足够染色体分裂相,5例由于细胞克隆数少而依靠传代培养获得结果。