Renalembryo 293 cell was co-transfected with the rAAV vector of plasmid pSSHG/NT4-GFP-Ant ,packaging plasmid pAAV/ Ad and helper adeno virus pasmidpFG140.The recombinant AAV viral stock was titrated by dot blot.

• 用腺病毒辅助质粒Pfg140、包装质粒pAAV/Ad及已构建的重组腺伴随病毒载体质粒,三质粒磷酸钙共沉淀法转染80%25融合的293细胞系,包装rAAV。