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After induced by IPTG, the recombine plasmid expressed the whole rat IDE and purified by the thrombin affinity chromatography. 重组质粒经IPTG诱导后，表达出了全长的大鼠IDE，并经过凝血酶层析柱进行纯化。
The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
The recombinant plasmid with the hammerhead ribozymegene was correct by digestion identification. 核酶基因重组子经酶切鉴定序列正确。
Recombinant plasmid pDAlllS was constructed by inserting 1.7kb aprA into the Nrul site of aveD. 将1.;7kb安普霉素抗性基因aprA插入aveD的NruI位点，得pDA1118。
Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in Human Gingival Fibroblast. 人釉原蛋白重组质粒Psec Taq2A-AMG在人牙龈成纤维细胞表达的研究
Objective Construct a recombinant plasmid pET28a-EDA-EDB,prepare the fusion EDA-EDB protein. 目的构建重组pET28a-EDA-EDB质粒,制备重组纤维连接蛋白EDA-EDB融合蛋白。
Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line. 目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。
A recombinant plasmid pUC-IL6 coding for IL6 was constructed by recombinant gene technique. 利用基因重组技术，构建含IL6基因的重组质粒pUC－IL6。
The recombinant plasmid of antisense TIMP 1 has a certain extent reverse effects on liver fibrosis. 反义TIMP 1表达质粒对肝纤维化有一定的逆转作用
The recombinant plasmid pBV220-Arr was construted successfully and the target protein Arresten can express in E. 成功构建Arresten基因重组质粒pBV220-Arr;并可在E.
The recombinant plasmid pPGVT3 derived from the vector pUC4 was unstable in the E. 从载体质粒pUC4衍生的重组质粒pPGVT3在大肠杆菌宿主DF2145中是不稳定的,以pPGVT3转化DF2145时在4o℃培养得不到转化子。
The recombinant plasmid was transformed into E. coli BL21(DE3), and E. coli BL21(DE3)/pET-LG3 was induced by IPTG. 用IPTG诱导,LG3蛋白在大肠杆菌BL21(DE3)中得到了表达,并经Ni-NTA层析柱获得纯化。
Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps. 结果构建了重组质粒pGEX-4T-CTB,CTB基因片段分子量约为376bp;
Authentication,PCR and DNA sequencing showed the recombinant plasmid of human MIA/CD-RAP was successfully constructed. 酶切电泳和DNA测序结果表明,成功地克隆了人黑素瘤M IA/CD-RAP cDNA。
The Arresten gene was screening successfully,and the recombinant plasmid pBV220-Arr was constructed successful. 成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达。
Method The recombinant plasmid pMD18-T-gG was constructed as HSV-2 DNA standard for quantitative analysis. 方法构建重组质粒 pMD18-T-gG作为标准品。
Methods Transform COS 7 cells with recombinant plasmid and detect instantaneously expressed product by ELISA. 方法质粒转染COS 7细胞 ,用ELISA法测定瞬时表达产物。
To construct a recombinant plasmid carrying the cholera toxin B subunit(CTB) gene and observe its expression in E. 目的构建霍乱弧菌肠毒素B亚单位(Cholera toxin B subunit,CTB)基因的大肠杆菌表达重组质粒,并观察其在大肠杆菌和双歧杆菌中的表达。
The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body. 对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。