Preculturing of immature cotyledon was beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn.

• 体细胞胚的脱水处理显著促进“中黄4号”体细胞胚的萌发。 未成熟子叶的预培养有利于根癌土壤杆菌感染子叶外植体体细胞胚的分化。