Porcine ovarian follicles were divided into healthy and atretic groups.Granulosa and thecal cells were isolated, and cultured with gonadotropin in the dual-chamber system.

• 方法：制备人胎盘羊膜双池培养系统，分离了猪卵巢颗粒细胞和卵泡膜细胞，并分为健康和闭锁两组，在促性腺激素刺激下协同培养。