Plasmid of HBV DNA dimer was transfected into a differentiated human hepatoma cell line(HepC2), and a stable HBV-producing cell line(z7-4) was set up which had capable of secreting HBcAg/HBeAg,viral nucleic acids and of releasing Dane-like particles.

• 用HBV DNA二聚体转染高度分化的人肝癌细胞株（HepG2），筛选出具有分泌病毒抗原、核酸以及释放42nm Dane颗粒能力的z7－4细胞株。