PCR analysis on 39 regenerated Km-resistant buds showed that 2 buds were PCR positive. The result indicated that gastrodia antifungal protein (GAFP) gene was integrated into the genome of the two Km-resistant buds.

• PCR检测有2个Km抗性芽呈稳定阳性,初步证明外源天麻抗真菌蛋白(GAFP)基因已整合到这2个Km抗性芽的基因组DNA中。