Optium fermentation medium and culture conditions were determined through Erlenmeyer flask liquid fermentation , ampliate liquid fermentation technique was also determined later.

• 通过茯苓的摇瓶液体发酵培养,确定了茯苓液体发酵的最适发酵培养基和最适培养条件,并进一步确定了最佳的放大培养工艺。