Only the marker amplified by S1431 was present in all 8 male individuals tested while absent from all 8 female individuals tested. It showed that it was a male marker closely linked to sex.

• 利用雌雄个体对18条可能与性别连锁的RAPD标记进行验证,发现引物S1431所扩增的约400bp谱带在8个雌性单株中都不出现,而在8个雄性单株中都出现,表明这是一条与雄性紧密连锁的RAPD分子标记。