One RING domain and two BIRs domain contained in IAP2 while one RING and one BIR domain contained in IAP3.iap2 and iap3 were ligated into expression vector pET-28a, then were transformed into BL21(DE3) competent cell respectively.

• 用表达载体pET28a-iap2转化宿主菌BL21(DE3)感受态细胞,挑取单菌落并接种于加卡那霉素的LB中,扩大培养后加入IPTG诱导表达,表达结果显示,含iap2基因载体的表达产物在电泳中出现一条约40kDa的表达带,与理论推测的蛋白分子量一致。