Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site.

• 以两条部分重叠长链引物P_2和P_3延伸产物为模板，P_1和P_4为正、反引物进行PCR扩增，获得了改造的抗菌肽基因SPCEMA(187bp)。