Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site.
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释义
以两条部分重叠长链引物P_2和P_3延伸产物为模板,P_1和P_4为正、反引物进行PCR扩增,获得了改造的抗菌肽基因SPCEMA(187bp)。
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