Objective: To develop an ELISA method of simple, high sensitivity and accuracy for direct quantitation of biotin in the yeast cultural media for gene transformation.

• 目的:采用一种简便、高灵敏度、高准确度的ELISA方法,对基因转殖可制造生物素的酵母菌发酵液中生物素含量的直接定量。