Objective: To construct eukaryotic expression vector pSecTag2/HygroB-DAF of human decay accelerating factor (DAF) and transfect NIH/3T3 cells after encapsulated by chitosan.

• 目的:构建人补体衰变加速因子(DAF)真核表达载体pSecTag2/HygroBDAF,并利用壳聚糖组装成纳米微粒复合体,转染小鼠NIH/3T3成纤维细胞。