Objective To establish the co-culture system in vitro and method of observation,so as provide the cell model for research of mammalian spermatogenesis.

• 目的建立体外长期共培养体系和观察方法,为精子发生过程的研究提供细胞模型。方法采用大鼠曲细精管生殖细胞及支持细胞共培养的方法,对睾丸生精细胞作显微镜观察。