Objective To develop a rapid and simple method of reverse transcription polymerase chain reaction (RT PCR) for the detection of mRNA from human cytomegalovirus (HCMV) immediate early (IE) gene.

• 目的建立快速简便的逆转录聚合酶链反应（RT?PCR）方法检测人巨细胞病毒（HCMV）即刻早期（IE）基因mRNA。