Objective To construct the recombinant plasmid of eukaryotic expression vector containing M-CSF gene and transfect it into the NIH3T3 cells to study whether resulting in the expression of M-CSF and EGFP fusion proteins.

• 目的构建真核表达重组体pEGFP-M-CSF,并鉴定其在小鼠成纤维细胞NIH3T3细胞能否正确表达。