Objective To clone toxin_coregulated pilus subunit A (tcpA) gene of vibrio cholerae O139 strain XJ93006 and construct the recombinant cloning vector of pNEB 193_tcpA.

• 目的 以新疆分离 0 13 9霍乱弧菌XJ93 0 0 6株的DNA为模板 ,自行设计引物克隆毒素协同调节菌毛亚单位A(tcpA)基因 (包括侧序列 )并构建重组载体。