OBJECTIVE To develop an HPLC-fluorescence metho d for determination trans tramadol and its active metabolite, O-demethyltrama dol (M1), in human serum, and study the pharmacokinetics.

• 目的 :建立人血清中反式曲马多 (transT)及其活性代谢物反式氧去甲基曲马多 (M 1)同时测定的HPLC荧光分析法并研究其人体药动学。