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Five mites from Ag positive mice, 50, 10 mites from Ag negative mice, 10, 5 free mites and 100 mg mouse lung of Ag positive mice were positive by nested RT PCR instead of negative by RT PCR. HFRSV抗原阳性鼠体恙螨5只组，HFRSV抗原阴性鼠体恙螨50只和10只组，游离螨10只和5只组，鼠肺HFRSV抗原阳性100mg组均未见有明显扩增带。
Study of detecting BCR/ABL fusion gene by using nest RT PCR 应用筑巢式RT-PCR检测BCR-ABL融合基因的探讨
Nested RT - PCR 巢式RT-PCR
In 47 serum samples which were HCV positive in ELISA,and 33(70.21%) were positive in RT nested PCR,39(82.98%) were positive in RT PCR DDH. 4 7份ELISA检测HCV抗体阳性血清标本 ;RT nested PCR检出阳性标本 33例 (70 .;2 1%25 );RT PCR DDH检出阳性标本 39例 (82
The swallows nested under the eaves. 燕子在屋檐下筑巢。
Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection. 方法通过RT PCR DDH ,RT nested PCR和HCVRNA DDH 3种方法检测HCV核酸 ,推断RT PCR DDH技术检测病毒核酸的特异性、敏感性和稳定性。
The bird is building a nest of straw and twigs. 这只鸟在用稻草和小树枝筑巢。
Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced. 方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。
RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
It proved that everything was a mare's nest. 结果证明一切都是场骗局。
Methods Plasma ETA was measured quantitively by RT PCR. 方法应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
The blackbird's nest contained four eggs. 黑鹂巢里有四个蛋。
The swallow has made a nest under our eaves. 燕子在我们的屋檐下垒了一个窝。
Reverse transcriptase (RT)-polymerase chain reaction PCR) of Cdx-2. CDX-2的逆转录酶（RT）聚合酶链反应（PCR）技术。
She nested the glass tumblers in a crate. 她把玻璃酒杯一个套一个放在箱子里。
The birds nested in the oak tree. 鸟在橡树上筑巢。
The redbreasts nested in the trees in our garden. 红胸鸟在我们庭院里的树上做巢。
A bird lays eggs in the nest it has built. 鸟将蛋下在自己所筑的巢里。
He went into illegal business to feather his nest. 他做非法生意以达到营私之目的。
Every bird likes its own nest the best. [谚]金窝银窝，不如自己的草窝。