您要查找的是不是:
- It is concordant with routine PCR method. 并与常规PCR法具有很好的一致性。
- The ISOObp promoter of PNZIP gene was cloned by adaptor PCR method. 因此,我们利用接头PCR技术克隆了PNZIP基因的启动子。
- The applications of Mullis' PCR method are already many. 原理简介:PCR技术的基本原理是DNA的半保留复制。
- Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established. 获取正常男性单个淋巴细胞和无DMD家族史的单个胚胎细胞,行DMD基因外显子17、19、44、45、48的五重巢式PCR,分析扩增成功率、假阳性率和假阴性率。
- A multiplex- PCR method for detection bovine materials in foodstuffs. 动物饲料中牛成分的复合PCR检测方法。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Methods The mRNA from normal lungs was used as template. By using the techniques of RT PCR, nest PCR and cloning, the pUC 19/SP A 6 A clone was established and sequenced. 方法以正常人肺组织mRNA为模板 ,采用逆转录反应 (RT) PCR、巢式 PCR及基因克隆技术 ,获得pUC19/SP A 6A克隆 ,并测序证实。
- Meanwhile, the PCR method is superior to immunohistochemistry in detecting HPV. 在检测上,PCR方法优于免疫组织化学染色,值得提倡和推广。
- Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+). 方法通过巢式PCR,从一份兰州地区无偿献血员血清中扩增TTV病毒的ORF2基因,并克隆到pGEM-T载体中,进行核苷酸序列分析。
- X gene (HBx), enhancer II (Enh2) and basic core promoter (Bcp) of HBV in sera from HCC patients were amplified by nest PCR , and sequenced and then compared with the sequences in Genbank. 对HCC患者血清的HBV的HBx、Enh2与Bcp基因的巢式PCR产物直接测序,与Genbank中已发表序列进行对比分析。
- Detection of Fetal SRY Gene from Maternal Blood by a Nested PCR. 利用套式PCR技术检测母血中胎儿SRY基因的研究
- Using PCR method, BBTV or CMV can be detected from the banana leaf tissue equivalent to 100 ng. 用PCR方法可从相当于100ng的叶片组织中检测到BBTV和CMV。
- Objective To establish a technique of single cell 5-plex nest PCR for Ducheme muscular dystrophy(DMD) detection and evaluate the possibility of using for preimplantation genetic diagnosis (PGD). 摘要目的:探索建立单细胞多重巢式PCR检测杜氏肌营养不良症(DMD)基因外显子17、19、44、45、48的技术,及应用于植入前遗传学诊断(PGD)的前景。
- Objective To develop a PCR method for the determination of porcine parvovirus(PPV). 目的建立猪细小病毒(PPV)PCR检测方法。
- Objective: To establish a technique of single cell 5-plex nest PCR for Ducheme muscular dystrophy (DMD) detection and evaluate the possibility of using for preimplantation genetic diagnosis (PGD). 目的:探索建立单细胞多重巢式PCR检测杜氏肌营养不良症(DMD)基因外显子17、19、44、45、48的技术,及应用于植入前遗传学诊断(PGD)的前景。
- The results prove that adapter ligation PCR method is workable to clone carnation CHS promoter. 然后利用衔接头PCR的方法进行了香石竹CHS启动子克隆的初步研究,得到了约500bp和800bp的PCR产物,目前克隆测序工作正在进行。
- This is to develop a rapid,sensitive and specific fluorescence PCR method detecting listeria monocytogenes in food. 发展一种快速、敏感、特异的荧光PCR方法检测食品中单核细胞增生李斯特氏菌。
- In order to improve diagmostic procedure of E. suis, the PCR method was established. 鉴于上述原因,为了建立特异准确的诊断方法,本研究开展了附红细胞体病的聚合酶链式反应(PCR)诊断方法的研究。
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- PCR method was considered as the gold standard, which showed the highest specificity (100%) and sensitivity (100%). PCR法是检测MRSA的金标准,特异性和灵敏度均为100%25。